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Gibco™ Viral Production Cells
Gibco Viral Production Cells are a derivative of the HEK 293F cell line and are a core component of the LV-MAX Lentiviral Production System (Cat. No. A35684).
Brand: Gibco™ A35347
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Description
Gibco Viral Production Cells are a derivative of the HEK 293F cell line and are a core component of the LV-MAX Lentiviral Production System (A35684). Viral Production Cells have been adapted to a special chemically-defined, serum-free and protein-free LV-MAX Production Medium (A3583401). They are maintained in suspension culture and will grow to high density in LV-MAX Production Medium. Viral Production Cells are highly transfectable by LV-MAX transfection reagent (A35348) and generate superior lentiviral production compared to polyethyleneimine (PEI)-based lentiviral production methods.
- Suspension culture cells facilitate high-throughput screening or scale-up
- Higher viral production levels than other suspension HEK 293 cells
- Rapid cell growth and high culture viabilities
Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in LV-MAX Production Medium and 10% DMSO. The cells should be thawed directly into LV-MAX Production Medium.
The cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable in volume from 96-deep well blocks to 2-L flasks and bioreactors. Lentiviral vector harvest is typically performed at two days post-transfection.
Specifications
| Store in liquid nitrogen until ready to use. Do not store the cells at -80°C. | |
| Kidney (Embryonic) | |
| LV-MAX™ Production Medium | |
| Viral Production Cells | |
| Dry Ice |
| Viral Production Cell-Line | |
| Suspension Cell Culture | |
| 1 x 107 | |
| 1 mL |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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