Goat anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 555, Invitrogen™

Description

To minimize cross-reactivity, the goat anti-rat IgG whole antibodies have been cross-adsorbed against serum proteins from mouse, horse, rabbit, bovine, and human to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only nonspecific-binding secondary antibodies are captured in the column, and highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experimentto eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. This antibody binds to heavy chains on rat IgG and light chains on all rat immunoglobulins, does not bind non-immunoglobulin rat serum proteins or serum proteins/IgG from mouse, horse, rabbit, bovine, or human.