H3K9me2S10phK27me2S28ph Monoclonal Antibody (51TA-2H12), Invitrogen™

Description

MA3-065 detects dimethyl-phospho-histone H3 Lys9/Lys27 pSer10/pSer28 in human samples and has successfully been used in western blot and immunoprecipitation applications. The MA3-065 immunogen is a synthetic peptide conjugated to ovalbumin corresponding to residues 180-195 of human TAF10 and only recognizes phosphorlayted pSer10 or pSer28 of Histone H3 when the adjacent Lys is dimethylated.

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.