Invitrogen™ HIF1A Human ELISA Kit

Description

The Human HIF1A ELISA quantitates Hu HIF1A in human serum, plasma, or cell culture lysates. The assay will exclusively recognize both natural and recombinant Hu HIF1A. Principle of the method The Human HIF1A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The Thermo Scientific HIF-1 alpha ELISA Kit contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate. This product contains reagents for analyte detection in 96 microwells.

Features of Thermo Scientific HIF-1 alpha ELISA Kits:

• Target: natural and recombinant human hypoxia-inducible factor 1-alpha (HIF1A)
• Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
• Measurement: absorbance at 450nm minus absorbance at 550nm
• Assay Range: 81.92 to 20,000 pg/mL
• Sensitivity: < 30 pg/mL
• Precision: intra-assay CV < 10%; inter-assay CV < 10%
• Sample Types: cell culture lysate, serum, plasma (EDTA, sodium citrate and heparin)
• Total Assay Time: 3.5 hours
• Sample Size: 50μL
• Specificity: specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A.

These HIF-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of hypoxia-inducible factor 1-alpha (HIF1A, also called BHLHE78, MOP1, PASD8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. .


For Research Use Only. Not for use in diagnostic procedures.