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Cytiva His SpinTrap™ Spin Columns
Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.
Brand: Cytiva 28-9321-71
This item is not returnable.
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Description
- Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
- One run in standard microcentrifuge takes approximatedly 10 min.
- Yields up to 750μg purified protein per column
- Allows direct purification of both clarified and unclarifed cell lysates
- Also useful for screening large numbers of small-scale bacterial lysates
- Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step
- Kit components (spin columns and buffer) can also be purchased separately
Medium Characteristics:
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- Stable (long-term) in the pH range 3-12
- Average particle size: 34μm
Order Info
Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)
Specifications
| His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC) | |
| 2 to 14 | |
| 4°C to 30°C | |
| Spin Column Kit |
| 1 Pk. His SpinTrap (28-4013-53) and 1 Pk. His Buffer Kit (11-0034-00) | |
| 6% Highly Cross-linked Agarose | |
| 1 Piece |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger||
Hazard Category
- Skin sensitiser Category 1
- Skin corrosion/irritation Category 1 C
- Reproductive toxicity Category 1B
Hazard Statement
- H317-May cause an allergic skin reaction.
- H314-Causes severe skin burns and eye damage.
- H360D-May damage the unborn child.
Precautionary Statement
- P201-Obtain special instructions before use.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P331-Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P310-Immediately call a POISON CENTER/doctor/.
- P305-IF IN EYES:Immediately call a POISON CENTER/doctor..
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Nickel, imidazole
- UNKNOWN AQUATIC-Contains 15% of components with unknown hazards to the aquatic environment.