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Thermo Scientific™ HisPur™ Cobalt Spin Columns, 0.2 mL
Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.
Brand: Thermo Scientific™ 89967
1526.50 SEK valid until 2024-12-20
Use promo code "21651" to get your promotional price.
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Description
Thermo Scientific™ HisPur Cobalt Resin is an immobilized metal chelate affinity (IMAC) support for specific, fast and gentle His-tagged purification.
Additional Information and Online Ordering
HisPur Cobalt Resins, Columns and Kits use a tetradentate chelating resin charged with divalent cobalt (Co2+)to enable specific, fast and gentle purification of histidine-tagged fusion proteins from a variety of sample types. HisPur Cobalt Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high throughput needs. With these options, high-quality His-tag protein purification is possible at nearly any scale. HisPur Cobalt Resin is superior to traditional nickel-based immobilized metal affinity chromatography (IMAC) systems. Compared to Ni-IDA and tetradentate Ni2+chelate resins, HisPur Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Use HisPur Columns and Cartridges to obtain high-purity His-tagged proteins with no metal contamination.Highlights:
High purity – obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
Low metal leaching – no metal contamination in eluted histidine-tagged protein sample
Versatility – purify proteins under native or denaturing conditions; compatible with cell lysis reagents and a variety of buffer additives
Flexibility – available as bulk resin or predispensed columns, chromatography columns and 96-well filter plates
Cost effective – resin can be reused several times or discarded after single use
Superior – performs better than other commercially available IMAC Resins
Available Formats:
- Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
- Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
- Purification Kits – complete kits in all three column sizes
- Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
- 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations
- Bulk and Custom – let us know your needs for bulk HisPur Cobalt Resin
Includes:
Kits contain HisPur Cobalt Columns, equilibration/wash buffer and elution buffer
Specifications
| 0.2 mL | |
| Store at 4°C | |
| HisPur™ | |
| Recombinant | |
| Spin Column | |
| Microcentrifuge, Spin Column |
| HisPur Cobalt Spin Columns, 0.2mL | |
| Liquid Suspension | |
| 25 Columns | |
| His-Tagged Protein | |
| Metal Chelate, Divalent Cobalt | |
| Affinity, Agarose Resin |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- HisPur Cobalt Spin Columns, 0.2 mL, 25 Columns
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H319-Causes serious eye irritation.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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