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Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 3 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Brand: Thermo Scientific™ 88229
2619.90 SEK valid until 2024-12-31
Use promo code "21651" to get your promotional price.
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Description
Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.
The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.
Highlights:
High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin
Versatile – purify proteins using native or denaturing conditions
Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges
Cost-effective – reuse the same batch of resin at least five times
Easy to use – pre-formulated buffers available for kit formats
Available Formats:
- Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
- Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
- Purification Kits – complete kits in all three column sizes
- Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
- Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin
Includes:
Kits contain HisPur Ni-NTA Columns
Specifications
| 1 mL/Column | |
| Sufficient For: Purifying up to 180 mg of His-tagged protein per column • HisPur Ni-NTA Spin Columns, 3 mL, 5 columns • Phosphate-buffered Saline (10X), 30 mL • Imidazole (2M), 25 mL • Column Plugs, 5 plugs Store at 4°C. |
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| 5 Column Kit | |
| Spin Column Kit | |
| 180 mg of His-Tagged Protein per Column | |
| Affinity, Agarose Resin |
| HisPur Ni-NTA Spin Purification Kit, 3mL | |
| HisPur™ | |
| His-Tagged Protein | |
| Divalent Nickel, Metal Chelate | |
| Spin Column |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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