missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Tango™ HTR2A-bla U2OS DA Assay Kit
Tango™ HTR2A-bla U2OS DA Assay Kit contain the human Serotonin Type 2A receptor (HTR2A) linked to a TEV protease site.
Brand: Invitrogen K1613
Additional Details : Weight : 0.05000kg
Description
- Gene Symbol: HTR2A
- Accession Number: NM_000621.2
- Cytoplasmic Tail: Tail from AVPR2 GPCR
- Population Status: Single Cell Clone Origin
- Includes: Tango™ HTR2A-bla U2OS DA cells, LiveBLAzer™-FRET B⁄G Loading Kit, 70 μg, Solution D, Tango™ HTR2A-bla U2OS DA Assay Protocol, LiveBLAzer™-FRET B⁄G Loading Protocol, Certificate of Analysis
- The Tango™ HTR2A-bla U2OS DA cells contain the human Serotonin Type 2A receptor (HTR2A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
- The Tango™ HTR2A-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ HTR2A-bla U2OS DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
Specifications
| NM_000621.2 | |
| Division-Arrested Cells | |
| Tail from AVPR2 GPCR | |
| HTR2A | |
| GeneBLAzer™ | |
| End Point | |
| HTR2A | |
| U2OS | |
| GPCR |
| Cell-based beta-arrestin recruitment | |
| K1613 Tango™ HTR2A-bla U2OS DA Assay Kit Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • Tango™ HTR2A-bla U2OS DA cells • LiveBLAzer™-FRET B⁄G Loading Kit, 70μg • Solution D • Tango™ HTR2A-bla U2OS DA Assay Protocol • LiveBLAzer™-FRET B⁄G Loading Protocol • Certificate of Analysis The Tango™ HTR2A-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| Fluorescent | |
| Single Cell Clone Origin | |
| GPCR (G-Protein Coupled Receptors) | |
| BLA (Beta-Lactamase) | |
| Division-Arrested Cells Assay Kit | |
| Tango™ |
For Research Use Only. Not for use in diagnostic procedures.