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Proteintech™ Human gp130 ELISA Kit
Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous gp130
Brand: Proteintech™ KE00176-96T
This item is not returnable.
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Description
- Recognizes human gp130
- gp130 (Glycoprotein 130, IL6ST, CD130, IL6-β) is ubiquitously expressed, signal-transducing receptor that serves as signal transduction unti for IL-6 family of cytokines (IL-6, IL-11, IL-27, LIF, OSM, CNTF, CT-1, CLC)
- Cytokines signal through gp130/Jak/STAT pathway
- Binding of IL-6 to IL6R induces gp130 homodimerization and formation of high-affinity receptor complex, which activates Jaks
- Causes phosyphorylation of gp130 tyrosine residues which activates STAT3
- gp130 is a Type I transmembrane protein
- Soluble form (sgp130) binds to sIL-6R/IL-6 complexes and prevents interactions with membrane-anchored gp130 on target cells
Process:
- Antibody specific for gp130 pre-coated onto microwells
- gp130 protein in samples captured by the coated antibody after incubation
- After extensive washing, another antibody of biotinylated specific for human gp130 is added to detect captured human gp130 protein
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correction wavelength set at 630 nm
Specifications
| NM_002184 | |
| 1.8 pg/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Human | |
| Human | |
| <10% | |
| Blue ice | |
| 4.5 hr. |
| 78.1 to 5000 pg/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 3572 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P40189 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 96TEST Human/Mouse SMN ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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