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Thermo Scientific™ Pierce™ Human MIP-1 alpha ELISA Kits
Measure levels of human macrophage inflammatory protein-1 alpha (MIP1a) in human serum, plasma and culture supernatant samples using 96-well plates.
Brand: Thermo Scientific™ EH2MIP1A
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Description
Assay MIP-1α (human macrophage inflammatory protein-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human MIP-1 alpha ELISA Kits.
These human MIP-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of macrophage inflammatory protein-1 alpha (MIP-1α) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant human MIP-1α (macrophage inflammatory protein-1 alpha)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 25.6 to 1000pg/mL
Sensitivity: < 2.5pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with human MIP-1β, MIP-1δ, MIP-1α, MIP-3β; mouse MIP-1α, MIP-1β, MIP-1γ, MIP-3β; or rat MIP-3α, MIP-I and MIP-II
Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
Specifications
| 25.6 to 1000pg/mL | |
| Human MIP-1 alpha ELISA Kit | |
| Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum | |
| 96 microplate wells | |
| Absorbance at 450nm minus absorbance at 550nm | |
| 1 Plate Kit | |
| 3 hr. |
| <2.5pg/mL | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| No significant cross-reaction with human MIP-1ß, MIP-1d, MIP-1a, MIP-3ß; mouse MIP-1a, MIP-1ß, MIP-1g, MIP-3ß; or rat MIP-3a, MIP-I and MIP-II | |
| Sample Diluent, 25mL, 30X Wash Buffer, 50mL, Anti-Human MIP-1a Precoated 96-well Strip Plate, 1 ea, Lyophilized Recombinant Human MIP-1a Standard, 2 vials, TMB Substrate Solution, 13mL, Adhesive Plate Covers, 6 ea, Streptavidin-HRP Reagent, 14mL, Stop Solution, 13mL, Biotinylated Antibody Reagent, 7mL | |
| Intra-assay CV <10%; inter-assay CV <10% | |
| Natural and recombinant human MIP-1a (macrophage inflammatory protein-1 alpha) |