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Molecular Probes™ BacLight™ Bacterial Membrane Potential Kit

BacLight™ Bacterial Membrane Potential Kit

Brand:  Molecular Probes™ B34950

Product Code. 10730964

  • 5000.00 SEK / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Contains DiOC2(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 μL of 500 μM solution in DMSO), and PBS (200 mL). Store in refrigerator (2–8°C) and protect from light.
Membrane Potential Assay
Bacterial Membrane Potential Kit
482⁄497
BacLight™
Wet Ice
Fluorescence
Flow Cytometer
Membrane Potential Probes
Tube(s)
1 kit
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number3006755 Certificate TypeCertificate of Analysis Date13/11/2024 Catalog NumberB34950
Lot Number2820933 Certificate TypeCertificate of Analysis Date02/02/2024 Catalog NumberB34950
Lot Number2530115 Certificate TypeCertificate of Analysis Date25/10/2022 Catalog NumberB34950
Lot Number2390590 Certificate TypeCertificate of Analysis Date07/10/2021 Catalog NumberB34950
Lot Number2277677 Certificate TypeCertificate of Analysis Date10/02/2021 Catalog NumberB34950
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For Research Use Only. Not for use in diagnostic procedures.