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Invitrogen™ Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3.

Brand:  Invitrogen™ 11363D

11670.50 SEK valid until 2024-12-31
Use promo code "21651" to get your promotional price.

Product Code. 10154833

  • 13730.00 SEK / Each
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For Research Use Only. All usage must comply with product instructions.


Includes: 2mL Antibody Mix Human CD4, 10mL Depletion MyOne; Dynabeads, 5mL Dynabeads CD25 and 2mL DETACHaBEAD™. These reagents are sufficient to process approx. 1 x 109 cells
This item is not returnable. View return policy

Description

Description

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.

Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells
• Treg cells can be expanded with Dynabeads™ CD3/CD28 Treg Expander and remain
Foxp3+.
• Treg cells can be used in any downstream applications (e.g., suppression assays)

Methodology
The isolation procedure includes the following steps:
• Negative isolation of untouched human CD4+ T-cells from PBMC.
• Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
• Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD™

Starting sample
PBMC

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

T Cells (Regulatory)
Cell Isolation Kit
Processes ∼1 x 109 cells total
DETACHaBEAD™, DYNAL™, Dynabeads™, FlowComp™
2 x 5 mL
Room Temperature
Human
Each Kit Contains:
• 2 mL Antibody Mix Human CD4
• 10 mL Depletion MyOne™ Dynabeads™
• 5 mL Dynabeads™ CD25
• 2 mL DETACHaBEAD™.

These reagents are sufficient to process ∼1 x 109 cells. Store components at 2°C to 8°C.
Depletion, then positive isolation
>95%
Research Grade
PBMC
1 x 108 PBMCs per isolation
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For Research Use Only. Not for use in diagnostic procedures.