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Invitrogen™ Dynabeads™ DNA DIRECT™ Blood Kit
The isolated DNA is free of RNA and contaminants that may inhibit PCR. The isolated DNA is of high integrity and high molecular weight, and PCR-results show excellent reproducibility in combination with high level of sensitivity.
Brand: Invitrogen™ 63102
Includes: Dynabeads (in Lysis buffer), red cell lysis buffer, washing buffer and resuspension buffer
Description
The isolated DNA is free of RNA and contaminants that may inhibit PCR. The isolated DNA is of high integrity and high molecular weight, and PCR-results show excellent reproducibility in combination with high level of sensitivity. The process of DNA isolation relies upon cell lysis to release DNA and adsorption of DNA to the surface of Dynabeads. This is followed by magnetic separation of intact DNA/Dynabeads complex and subsequent washing to remove any residual contaminants and potential PCR inhibitors from isolated DNA. Finally, complex is resuspended for direct use in downstream PCR-reactions. Alternatively, DNA may be eluted from Dynabeads following short incubation at 65°C. Multiple samples may be handled in parallel and isolation process can be automated.
- Cost-effective, simple and efficient single-tube procedure, completed in only 15 minutes
- Supports 100 isolations from 100 μL of blood per sample
- Sufficient for at least 100 PCR amplifications
- Protocol can be modified to allow isolation from 500 μL blood samples, providing enough template DNA for 1,000 PCR amplifications
- Isolation of PCR-ready genomic DNA directly from blood
- <100 μL Blood Samples (Small-Scale), 100μL - 1mL Blood Samples ( Medium-Scale)
Order Info
Shipping Condition: Room Temperature
Specifications
Store in refrigerator (2–8°C). | |
Blood | |
Not High-throughput Compatible (Manual) | |
DNA DIRECT™, DYNAL™, Dynabeads™ | |
100 preps | |
Genomic DNA |
Magnetic Bead | |
Sequencing | |
< 100 μL Blood Samples (Small-scale), 100 μL to 1 mL Blood Samples (Medium-scale) | |
Blood Kit | |
Room Temperature |
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For research use only. Not for use in diagnostic procedures.