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Invitrogen™ Dynabeads™ M-270 Epoxy

Dynabeads™ M-270 Epoxy beads are 2.8 μm superparamagnetic beads containing surface epoxy groups.

4540.00 SEK - 16120.00 SEK

Specifications

Product Type Epoxy Bead
Elution Volume Min 5 μL
Format Freeze Dried
Product Line DYNAL™, Dynabeads™
Purity or Quality Grade Research Grade
View More Specs

Includes: 60mg Dynabeads M-270 Epoxy, approx. 6.7 x 107 beads/mg, supplied as a freeze-dried powder.

Products 2
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Product Code Brand Quantity Price Quantity & Availability  
10512625
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Invitrogen™
14301
60 mg
4540.00 SEK
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10727313
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Invitrogen™
14302D
300 mg
16120.00 SEK
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Description

Description

Dynabeads™ M-270 Epoxy beads are 2.8 μm superparamagnetic beads containing surface epoxy groups. The beads covalently bind primary amino and sulfhydryl groups in proteins and peptides, making them ideal for coupling antibodies, peptides, intact proteins, and functional enzymes. These hydrophylic, neutral pH beads exhibit extremely low nonspecific binding of proteins and dyes, which reduces the need for blocking agents. Also, Dynabeads™ M-270 Epoxy Beads do not contain Tween™ detergent, and so are ideal for use in mass spectrometry (MS) analysis.

• Ideal for immunoprecipitation (IP) of proteins and protein complexes
• Gentle, yet rapid magnetic separation and short incubation times allow identification of transient and labile complexes
• Covalent antibody-coupling to the Dynabeads™ avoids co-elution of the antibody with the target protein

Adaptable for various applications
Fast surface binding kinetics and rapid magnetic separation characteristics make Dynabeads™ M-270 Epoxy beads the ideal choice for applications such as immunoprecipitation/purification of proteins and protein complexes, coupling functional enzymes to the bead surface for downstream assays, and identifying protein binding partners. Captured proteins and protein complexes are easily separated, washed, and eluted using a DynaMag™ magnet, and the magnetic separation properties of the Dynabeads™ .

Coupling procedure
Covalent coupling is performed overnight by incubating the desired ligand (commonly antibodies, peptides, or proteins) with Dynabeads™ M-270 Epoxy beads. The binding capacity varies depending on the ligand used, but is typically 5–10 μg IgG per milligram of beads. Coupling occurs at neutral pH and high salt concentration. The coupling reaction can be performed over a broad range of temperatures to match the stability characteristics of your ligand.

Upon completion of the ligand coupling step, the reactive moieties on the Dynabeads™ M-270 Epoxy beads are deactivated to prevent further coupling. Once the coupling reaction is completed, these nonporous, hydrophilic, pH neutral beads exhibit extremely low nonspecific binding.

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Epoxy Bead
Freeze Dried
Research Grade
Room Temperature
2.8 μm
5 μL
DYNAL™, Dynabeads™
Any Sample Type
Epoxy
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Safety and Handling

Safety and Handling

missing translation for 'shelfLife' : 24 months from date of manufacture

SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.