missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Biochemical Reagents
  4. DNA Reagents
  5. PCR Cloning Kits
  6. 10093392

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells

Enables rapid cloning of one or more genes into virtually any protein expression system

Brand:  Invitrogen™ 12535037

6659.40 SEK valid until 2024-12-31
Use promo code "21651" to get your promotional price.

Product Code. 10093392

  • 10090.00 SEK / 1 set

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers

For Research Use Only. All usage must comply with product instructions.


Includes: Gateway BP Clonase II enzyme mix and reaction buffer, Gateway pDONR;221 or pDONR;/Zeo vector, M13 sequencing primers, PEG/Mg2+ solution, proteinase K, pEXP7-tet positive control, and OmniMAX2 Competent Cells.
This item is not returnable. View return policy

Description

Description

  • Designed to create entry clone following PCR with attB-modified custom primers
  • With entry clone, recombine gene of interest into variety of expression vectors adapted for use in Gateway technology
  • PCR product is directionally cloned with efficiencies typically >99%
  • Rapid, efficient, directional cloning of PCR products
  • Versatility to use any amplification enzyme
  • Cloning without need for restriction endonucleases or ligase
  • Selection of entry clones with kanamycin or Zeocin™
  • In addition, the pDONR221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use

Cloning,Gateway Cloning

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Gateway™
Gateway™, OmniMAX™, pDONR™
20 reactions
The PCR Cloning Kit with Gateway™ Technology includes Gateway™ BP Clonase™ II enzyme mix and reaction buffer, Gateway™ pDONR™221 or pDONR™/Zeo vector, M13 sequencing primers, PEG/Mg2+ solution, proteinase K, pEXP7-tet positive control, and One Shot™ OmniMAX™2 Competent Cells. Store competent cells and enzyme mix at -80°C. Store all other components at -20°C.
PCR Cloning System
pDONR
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.