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Invitrogen™ pTrcHis2 A, B, & C Bacterial Expression Vectors

Offer enhanced translation initiation and high-level expression in E. coli

Brand: Invitrogen™ V36520

Product Code. 10554383

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For Research Use Only. All usage must comply with product instructions.

Includes: All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided.

This item is not returnable. View return policy

Description

These vectors feature high-level regulated transcription from the trc promoter
Enhanced translation efficiency of eukaryotic genes in E. coli
The lacO operator and lacIq repressor gene for transcriptional regulation in any E. coli strain
This particular vector offers C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an anti-HisG antibody
C-terminal Xpress epitope for easy detection with an anti-Xpress antibody
Enterokinase cleavage site for removal of fusion tag

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Constitutive or Inducible System	Inducible
Promoter	Trc, lacO
Selection Agent (Eukaryotic)	None
Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Protein Tag	Thioredoxin
Quantity	20 μg

Inducing Agent	IPTG
Product Type	Bacterial Expression Vector
Content And Storage	All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided. Store vectors at -20°C. Store stab at room temperature. The vector is guaranteed stable for 6 months when properly stored.
Cleavage	EK (Enterokinase) Recognition Site
Cloning Method	Restriction Enzyme/MCS
Vector	pTrc

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For Research Use Only. Not for use in diagnostic procedures.

Description