Invitrogen™ One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli

Description

One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli are the fastest growing chemically competent cells with a doubling time approximately 50 minutes. Mach1 T1^R colonies are clearly visible within eight hours of plating the transformation mix (ampicillin selection), enabling you to plate and pick colonies the same day. Plasmid DNA can be prepared four hours after inoculating a single, overnight-grown colony in the selective media of choice (with any antibiotic). Mach1 T1^R Chemically Competent E. coli cells can achieve transformation efficiency of >1 x 10⁹ cfu/μg plasmid DNA.

The Mach1 T1^R strain is derived from a non-K-12, wild-type W E. coli strain (ATCC #9637) that had been selected due it’s growth speed and genetically manipulated to possess the most useful cloning strain features. The strain was engineered to carry the tonA (also known as fhuA) mutation that confers resistance to T1 and T5 phage infection. The tonA genotype offers added security to valuable clones and libraries. The Mach1 T1^R strain has the lacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. It also carries the recA1398 mutation that helps reduce the rate of recombination while propagating plasmid DNA, the endA1 mutation that increases plasmid DNA yield and quantity, and hsdR restriction system deletion to allow genomic DNA cloning.

One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli offer:
• Transformation efficiencies of >1 x 10⁹ cfu/μg plasmid DNA
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage 
• lacZ∆M15 for blue-white color screening of recombinants
• hsdR mutation for efficient transformation of unmethylated DNA from PCR applications
• recA1398 mutation for reduced occurrence of homologous recombination in cloned DNA
• endA1 mutation for increased plasmid yield and quality

Easy-to-use One Shot format 
Mach1 T1 Phage-Resistant Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, helping save time and prevent contamination.

Genotype
F^– φ80lacZΔM15 ΔlacX74 hsdR(r_K–, m_K+) ΔrecA1398 endA1 tonA

Find the strain and format that you need 
• We offer other strains in chemically competent and electrocompetent cell formats to meet your specific needs.
• Mach1 T1^R and other strains are available in MultiShot formats for high throughput applications.