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Invitrogen™ PureLink™ 96 HQ Plasmid DNA Purification Kit
The PureLink HQ 96 Plasmid DNA Purification Kit is designed for high-throughput isolation of high-quality plasmid DNA from bacterial cells.
Brand: Invitrogen™ K210096
Includes: The PureLink HQ Mini Plasmid DNA Purification Kit includes sufficient PureLink columns, collection tubes, elution tubes, and reagents for 100 purifications.
Description
The PureLink HQ 96 Plasmid DNA Purification Kit is designed for high-throughput isolation of high-quality plasmid DNA from bacterial cells. It can be used with a vacuum manifold or a centrifuge and is compatible with automated liquid handling workstations.
The PureLink HQ 96 Plasmid DNA Purification Kit offers the following advantages:
• Ability to isolate up to 10 μg plasmid DNA per well
• Designed to isolate high-quality plasmid DNA in 30-45 minutes
• Minimal genomic DNA contamination in the purified plasmid DNA
• Compatible with automated liquid handling workstations
• Reliable performance of the purified plasmid DNA in downstream applications such as mammalian transfection
Process overview
Bacterial cells are harvested and resuspended in Resuspension Buffer with RNase. The cells are lysed using an alkaline/SDS cell lysis procedure. The lysate is then neutralized and conditions are adjusted for subsequent binding. After lysate clarification using the PureLink HQ 96 Clarification Plate, the lysate is processed through the PureLink HQ 96 Binding Plate. The DNA binds to the silica-based membrane in the plate and impurities are removed by washing with Wash Buffer. The DNA is then eluted in low salt Elution Buffer or water.
Order Info
Shipping Condition: Room Temperature
Specifications
| Sufficient reagents are provided in the kit to perform 384 (4 x 96) isolations. Reagents include: • Resuspension Buffer (R1) • Lysis Buffer (L1) • Neutralization/Binding Buffer (B1) • Wash Buffer (W1) • Elution Buffer; 10 mM Tris-HCl, pH 8.5 (E1) • RNase A • PureLink HQ 96 Clarification Plate • PureLink HQ 96 Binding Plate • Receiver Plate • Adhesive Foil for plates |
|
| Silica Plate | |
| Plasmid DNA | |
| PCR, Transfection, Sequencing, Transformation, Cloning | |
| 4 x 96 Preps | |
| PureLink™ | |
| 4 x 96 preps | |
| Plasmid DNA |
| 96-well Plate | |
| Bacterial Culture | |
| Automated Liquid Handling Workstation | |
| High-throughput Compatible | |
| < 100 μg (Small-Scale) Plasmid DNA | |
| Plasmid DNA Purification Kit | |
| Room Temperature | |
| 30 to 40 min. |
For Research Use Only. Not for use in diagnostic procedures.