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  5. Invitrogen™ T4 DNA Ligase (1 U/μL)

Invitrogen™ T4 DNA Ligase (1 U/μL)

Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA

660.00 SEK - 8050.00 SEK

Specifications

Concentration 1 U/μL
Enzyme Ligase
Product Type T4 DNA Ligase
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Includes: Vial of 5X reaction buffer (250mM Tris-HCl (pH 7.6), 50mM MgCl2, 5mM ATP, 5mM DTT, 25% (w/v) polyethylene glycol-8000)

Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10124752
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Invitrogen™
15224017 		100 U
660.00 SEK
Each
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10534613
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Invitrogen™
15224025 		500 U
2265.00 SEK
Each
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11558726
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Invitrogen™
15224090 		4 x 500 U
8050.00 SEK
Pack of 4
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Description

Description

T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3'hydroxyl and 5'phosphate termini. Single-stranded nucleic acids are not substrates for this enzyme.

  • Optimizes ligation in 5 minutes
  • Source: Purified from E. coli lambda lysogen NM989
  • Performance and quality testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested
  • Unit definition: One unit catalyzes exchange of 1nmol 32P-labeled pyrophosphate into ATP in 20 min. at 37°C. One unit is equal to approximately 300 cohesive-end ligation units
  • Unit reaction conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl2, 10mM DTT, 66μM ATP, 3.3μM 32P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min. at 37°C

ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics & Noncoding RNA Research, Restriction Enzyme Cloning

Order Info

Shipping Conditions: Dry ice

Specifications

Specifications

1 U/μL
Ligase
T4 DNA Ligase
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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