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Invitrogen™ T7 RNA Polymerase

DNA-dependent RNA polymerase with high specificity for bacteriophage T7 promoter sequences

Brand:  Invitrogen™ 18033100

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Product Code. 11528906

  • 5050.00 SEK / Pack of 2

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For Research Use Only. All usage must comply with product instructions.


Includes: T7 RNA Polymerase is supplied with a vial of 5X reaction buffer [200mM Tris-HCl (pH 8.0), 40mM MgCl2 , 10mM spermidine-(HCl)3 , 125mM NaCl], vial of 100mM DTT.
This item is not returnable. View return policy

Description

Description

Synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available.

  • Application: Synthesis of labeled and unlabeled RNA transcripts.
  • Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid
  • Performance and Quality Testing: 3' and 5' exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction
  • Unit Definition: One unit hydrolyzes 1nMol of ribonucleotide into acid-precipitable material in 1 hr at 37°C using a T7 transcription vector as template
  • Unit Reaction Conditions: 40mM Tris-HCl (pH 8.0), 25mM NaCl, 8mM MgCl2, 2mM spermidine-(HCl)3, 5mM DTT, 0.4mM ATP, 0.4mM CTP, 0.4mM GTP, 0.4mM UTP, 1μCi [3H]GTP, 1μg SphI-cut pT7L13, and enzyme in 50μL for 10 min. at 37°C

Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, Protein Expression, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Wet Ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

T7 RNA Polymerase (50 U/μL) is supplied with a vial of 5X reaction buffer [200 mM Tris-HCl (pH 8.0), 40 mM MgCl2 , 10 mM spermidine-(HCl)3 , 125 mM NaCl], vial of 100 mM DTT. Store at -20°C.
T7 RNA Polymerase
T7
2 x 2500 U
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For Research Use Only. Not for use in diagnostic procedures.