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Thermo Scientific™ Klenow Fragment (10 U/μL)

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

750.00 SEK - 2985.00 SEK

Specifications

Concentration 10 U/μL
Polymerase DNA Polymerase I
Product Type Klenow Fragment
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Products 2
Product Code Brand Concentration Quantity Price Quantity & Availability  
Product Code Brand Concentration Quantity Price Quantity & Availability  
10130800
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Thermo Scientific™
EP0051
10 U/μL 300 U
750.00 SEK
300 units
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10795071
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Thermo Scientific™
EP0052
10 U/μL 1500 U
2985.00 SEK
1500 units
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Description

Description

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.

Highlights

  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
  • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers

Applications

  • DNA blunting by fill-in 3'-overhangs
  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA
  • DNA sequencing by the Sanger method
  • Site-specific mutagenesis of DNA with synthetic oligonucleotides
  • Second strand synthesis of cDNA
Specifications

Specifications

10 U/μL
DNA Polymerase I
Klenow Fragment
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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