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Lysis buffer BQ1, Macherey Nagel Bioanalysis™

Product Code. 10020522
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Quantity:
125 mL
This item is not returnable. View return policy
This item is not returnable. View return policy
Flexible handling of multiples of 8 samples is achieved by use of a reusable column holder. Parallel lysis of large sample numbers can be performed in standard microcentrifuge tubes or in 96 well blocks alternatively.NucleoSpin™ Blood QuickPure binding strips are to be used in a centrifuge with deep buckets allowing processing of NucleoSpin™ 8 well strips stacked on 96 well blocks. For further information contact our Technical Support Team.The method is based on a specially treated silica membrane which speeds up the procedure by a combined washing/drying step. The number of washing and drying steps is reduced from 3 to only 1, therefore hands-on time is minimised.Lysis is achieved by incubation of whole blood in a solution containing large amounts of chaotropic ions in the presence of proteinase K. Appropriate conditions for binding of DNA to the silica membrane of the NucleoSpin™ Blood QuickPure binding strips are created by addition of ethanol to the lysate. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer and can be used directly for PCR*, Southern blotting or any kind of enzymatic reaction. The kit allows isolation of 4μg to 6μg of pure genomic DNA from 200μL whole blood with an A260/280 ratio between 1.60 and 1.90 and a typical concentration of 40ng/μL to 60ng/μL.Kit components: NucleoSpin™ Blood QuickPure binding strips, buffers, proteinase K, accessories.
  • Silica membrane technology
  • Manual processing
  • Yield: 4μg to 6μg
  • Sample material: human or animal whole blood (200μL/300μL†)
  • Elution volume: 7.5μL to 100μL
  • Binding capacity: 60μg
  • Preparation time: 60min/12 strips
Ultra-fast, small-scale purification of genomic DNA from whole blood, serum, plasma or other body fluids.
† For preparation of 300μL samples increased volumes of lysis buffer BQ1 required.

Specifications

Quantity 125 mL
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Signal Word
  • Warning
Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
Supplemental information
  • MIXTURE LIST-Contains : guanidine hydrochloride
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