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Description
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle’s salts.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco™ MEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ MEM product made in our Grand Island facility. This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Specifications
ISO 13485 standards, FDA | |
MEM Medium, 500mL, 1 X Concentration, Liquid Form, With L-glutamine, Phenol Red, 7 to 7.4 pH, 12 Months Shelf Life, 280 to 320mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture | |
Liquid | |
Glutamine, Phenol Red | |
Mammalian cell culture | |
10 x 500 mL |
2 to 8°C | |
1 X | |
MEM Medium | |
No HEPES | |
7 to 7.4 | |
For in-vitro diagnostic use |