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Proteintech™ Mouse IL-4 ELISA Kit
KE10010 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL4 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL4.
Brand: Proteintech™ KE10010-96T
This item is not returnable.
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Description
- The assay recognizes mouse IL4. An antibody specific for mouse IL4 has been pre-coated onto the microwells.
- The mouse IL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL4 is added to detect the captured mouse IL4 protein.
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent.
- Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
- Interleukin-4 (il-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells.
- It promotes the proliferation and differentiation of antigen presenting cells. Il4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE.
- This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis.
- Il4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.
Specifications
| NP_067258 | |
| 1 pg/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Mouse | |
| Mouse | |
| <10% | |
| Blue ice | |
| 4.5 hr. |
| 7.8 to 500 pg/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 16189 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P07750 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 96TEST Human Galectin-3 ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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