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Proteintech™ Mouse TNF-alpha ELISA Kit
KE10002 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNF-α ELISA kit is to be used to detect and quantify protein levels of endogenous TNF-α.
Brand: Proteintech™ KE10002-96T
This item is not returnable.
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Description
- The assay recognizes mouse TNF-α. An antibody specific for TNF-α has been pre-coated onto the microwells. The TNF-α protein in samples is captured by the coated antibody after incubation.
- Following extensive washing, another antibody of biotinylated specific for TNF-α is added to detect the captured TNF-α protein.
- For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
- TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily.
- It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation.
- It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons.
- It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Mouse and human TNF-alpha share 79% amino acid sequence identity.
- Unlike human TNF-alpha, the mouse form is glycosylated.
- In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles.
Specifications
| NP_001265530 | |
| 1 pg/mL | |
| Sandwich ELISA, Double Antibody | |
| Plasma, Serum | |
| Mouse | |
| Mouse | |
| <10% | |
| Blue ice | |
| 4.5 hr. |
| 7.8 to 500 pg/mL | |
| ISO 13485 | |
| Coated ELISA Kit | |
| DAB | |
| 21926 | |
| Solid | |
| <10% | |
| -20°C to +4°C | |
| P06804 |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 96TEST Mouse TNF-alpha ELISA Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P234-Keep only in original container.
- P264-Wash skin thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P330-Rinse mouth.
- P362-Take off contaminated clothing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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