Invitrogen™ AcTEV™ Protease

Description

An improved version of Tobacco Etch Virus (TEV) AcTEV™ Protease is highly site-specific, highly active and significantly more stable than native TEV protease, resulting in enhanced long-term activity.

• Highly specific cleavage activity
• Enhanced enzyme stability for prolonged protease activity
• Activity over a broad temperature (4°C to 30°C) and pH (6.0 to 8.5) range
• Six-histidine sequence to facilitate its removal from the digested protein sample
• Greater than 85% single-band purity with no nonspecific protease contamination

• Application
• Incubation with AcTEV™ Protease releases the protein of interest from the fusion tag
• This is an effective way to remove solubility, secretion, detection, and purification tags from recombinant protein
Enzyme specifications
• Purified from E. coli expressing the AcTEV™ Protease gene
• Unit definition
• One unit of AcTEV™ Protease cleaves 85% of a 3μg control substrate in 1 hr at 30°C
• Unit reaction conditions
• 50mM Tris-HCl (pH 8.0), 0.5mM EDTA, 1mM DTT, 3μg control substrate, and 1 unit enzyme in 30μL for 1 hr at 30°C AcTEV™ Protease is functionally tested for the absence of any nonspecific protease activity

GST-Tagged Protein Purification, His-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis