The Dynabeads™ Antibody Coupling Kit is intended for the coupling of an antibody or protein to the surface of Dynabeads™ M-270 Epoxy beads. The coupled beads and supplied buffers can then be used for experiments such as immunoassays, immunoprecipitation (IP), Co-IP of protein complexes, chromatin IP (ChIP), RNA-IP (RIP), cell isolation, etc. The Dynabeads Epoxy beads in this kit do not contain Tween™ detergent making them suitable for mass spectrometry (MS) analysis. The kit includes the buffers needed for efficient antibody coupling and washing steps. Note that the SB buffer supplied in the kit does contain Tween™ detergent, which means the buffer will need to be replaced with standard TBS or PBS buffer if the kit is to be used for MS.
Benefits of Dynabeads Antibody Coupling Kit include: • Convenience—contains optimized buffers and epoxy-activated Dynabeads magnetic beads • Efficiency—easy-to-use protocol with minimal hands-on time • Flexibility—allows coupling of any antibody of your choice • Robustness—covalent antibody coupling to the beads avoids co-elution of the antibody with the target proteins
Efficient and easy coupling With this kit antibodies are covalently coupled to Dynabeads M-270 Epoxy beads, minimizing the risk of bound antibodies contaminating the final eluate. Dynabeads M-270 Epoxy beads exhibit extremely low background binding and do not require blocking before use. Other proteins such as lectins and enzymes may also be coupled using the same beads, buffers, and protocol (depending on the stability and functionality of the protein/ligand you are using).
Magnetic bead-based separation offers easy handling Captured proteins and protein complexes are easily separated, washed, and eluted using a DynaMag magnet due to the magnetic properties of the Dynabeads M-270 Epoxy beads. The supplied buffers are optimized to give consistent and reliable results. In addition to Dynabeads M-270 Epoxy beads, the kit includes five different binding, washing, and elution buffers. Target specific antibody is required for coupling, but not included.
Automation-ready for use with KingFisher purification systems The 2.8-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher sample purification systems or liquid handlers.
Commercial supply Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads Antibody Coupling Kit in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or read more on our Dynabeads OEM page.
Customers who viewed this item also viewed
This information does not imply a recommendation or representation of any kind and any action taken upon the information provided is strictly at your own risk. The products shown have not been qualified by Fisher Scientific for any particular purpose, application or use and it is the responsibility of the purchaser to ensure that the performance of the product is suitable for the purchasers specific use or intended application.
