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Invitrogen™ RANTES Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHRNTS2
Description
The Human RANTES ELISA quantitates Hu RANTES in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu RANTES. Principle of the method The Human RANTES solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Recognizes natural and recombinant IP-10.Specifications
P13501 | |
2 pg/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
CCL5 | |
<10% | |
HRP | |
RUO | |
2°C to 8°C | |
1 hr. 20 min. |
51.2-2000 pg/mL | |
Biotin | |
Plasma, Serum, Supernatant, Urine | |
Chromogenic Detection | |
6352 | |
<12% | |
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
2 x 96 Tests | |
Plasma, 1 μL; Serum, 1 μL; Supernatant, 50 μL; Urine,50 μL | |
Human | |
3 hr. |