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Macherey-Nagel™ Binding Buffer NTB
Binding Buffer NTB to be used with NucleoSpin Gel and PCR Clean‐up kits, for clean up of SDS containing samples.
Brand: Macherey-Nagel™ 740595.150
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Description
NucleoTrap™ is a silica matrix based kit for isolation of highly pure DNA fragments from agarose gels and aqueous solutions (desalination). The kit can also be used for the removal of nucleotides and/or biotin after labelling reactions as well as for the removal of enzymes. By means of a specially activated spherical silica matrix it is possible to extract DNA fragments from agarose gels or solvents in a very mild manner, without the use of organic solvents. Nucleic acids are bound to the NucleoTrap™ matrix in the presence of a chaotropic salt. The hydrate shell of the nucleic acids is destroyed by this binding buffer, thus facilitating adsorption to the specially treated NucleoTrap™ matrix. A washing step is followed by the elution of DNA with low-salt elution buffer or distilled water. Kit components: NucleoTrap™ suspension, buffers.- Silica matrix technology
- Fragment size: 20bp to 20kb
- Sample material: 20mg to 600mg agarose gel
- Elution volume: 20μL to 50μL
- Binding capacity: 6μg/10μL suspension
- Typical Recovery: 50% to 90%
- Preparation time: 60 min./6 preps
- Format: silica bead suspension For extraction of DNA from agarose gels, suitable, for example, for subsequent cloning sequencing, PCR, restriction analysis. Support protocol for concentration and desalination of reaction mixtures.
Specifications
| 150 mL | |
| For Clean-up of SDS-Containing Samples | |
| Binding Buffer NTB |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- buffer NTB
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H319-Causes serious eye irritation.
- H336-May cause drowsiness or dizziness.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash with water thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P330-Rinse mouth.
Supplemental information
- MIXTURE LIST-Contains : guanidine hydrochloride, propan-2-ol
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