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Macherey-Nagel™ NucleoSpin™ RNA Plus Kit
Efficient and quick RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms. Macherey-Nagel™ NucleoSpin™ RNA Plus Kit includes the NucleoSpin™ gDNA Removal Column, a spin column, which quickly and effectively removes genomic DNA contamination without DNase digestion.
Brand: Macherey-Nagel™ 740984.10
This item is not returnable.
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Description
During the isolation of RNA, it is imperative to prevent the degradation of the RNA. In the NucleoSpin™ RNA Plus method, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases. The lysate is added to the gDNA Removal Column to clarify the lysate and to remove contaminating gDNA. After the addition of the Binding Solution to the flow-through, the RNA is bound to the RNA Plus Column. Two subsequent wash steps remove salts, metabolites, and macromolecular cellular components. High quality RNA is eluted with RNase-free H2O.
- Includes NucleoSpin™ gDNA Removal Column: no time consuming rDNase digestion
- Convenient handling: lysate clearing and gDNA removal with one column in one step
- New efficient lysis buffer: no β-mercaptoethanol or TCEP necessary
- Downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
- Kit contents: NucleoSpin RNA Plus Columns, NucleoSpin gDNA Removal Columns, Collection Tubes, buffers
Specifications
| <109 Cells | |
| Lysis Buffer | |
| Silica Membrane System (Two Columns) | |
| <1 x 107 Cultured Cells, <1 x 109 Bacterial Cells, <1 x 108 Yeast Cells, <30 mg Human/Animal Tissue | |
| NucleoSpin™ RNA Plus Columns with Collection Tubes, NucleoSpin™ gDNA Removal Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers | |
| NucleoSpin RNA Plus kit | |
| Yes | |
| 20 min./6 Reaction |
| Room temperature (18-25°C) | |
| Mini Spin Column | |
| Elution volume: 30–120μL | |
| RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms | |
| 10 | |
| 1 Kit | |
| Fragment size: >200 nt | |
| HeLa cells 5 x 106: 40-60μg Mouse liver 20mg: 80-100μg Mouse kidney 20mg: 40-70μg Mouse spleen 5mg: 30-60μg |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Supplemental information
- Warning
Hazard Category
- Flammable liquid Category 3
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H412-Harmful to aquatic life with long lasting effects.
Supplemental information
- MIXTURE LIST-Contains : ethyleneglycol diacetate / guanidinium thiocyanate /guanidine hydrochloride, ethanol