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Lonza™ P3 Primary Cell 4D-Nucleofector™ X Kit S

For efficient transfection of specific primary cells, e.g., human T cells, CD34 or rat neurons, in the 4D-NucleofectorTM X Unit (20 μL format).

Brand: Lonza™ V4XP-3032

Product Code. 12618553

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Description

High transfection efficiencies for primary cells.
Cells maintain functionality post transfection.
Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System.
Applications:
- Transfection of cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells.
- Medium throughput applications.
- Optimization of transfection conditions, e.g., substrate titrations.
This kit has been determined to be optimal for transfecting following cell types:
- B cell (human)
- CD34+ cell (human)
- Chondrocyte (human)
- DC (human)
- DC (mouse), mature
- ES (human), H9
- ES (mouse)
- Hepatocyte (human)
- HMEC (human)
- Keratinocyte (human)
- Macrophage (human)
- Monocyte (human)
- Neuron (rat), brain Hi/Cx
- NHBE (human)
- RPE (human)
- T cell (human), stim.
- T cell (mouse), unstim.

Specifications

Color	Clear
For Use With (Equipment)	4D-Nucleofector™ X Unit (20 μL format)
Includes	P3 Primary Cell Nucleofector™ Solution, Supplement 1, pmaxGFP™ Vector (1 μg/μL in 10 mM Tris pH 8.0), 16-well Nucleocuvette™ Strips
pH	7.1 to 7.3

Vector	pmaxGFP™
For Use With (Application)	Efficient transfection of specific primary cells, e.g., human T cells, CD34 or rat neurons.
No. of Reactions	32
Quantity	2 x 0.675 mL P3 Primary Cell Nucleofector™ Solution, 2 x 0.15 mL Supplement 1, 50 μg pmaxGFP™ Vector (1 μg/μL in 10 mM Tris pH 8.0), 2 x 16-well Nucleocuvette™ Strips

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