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Thermo Scientific™ Phire Green Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.
1302.00 SEK - 5530.00 SEK
Specifications
GC-Rich PCR Performance | Medium |
---|---|
Polymerase | Phire Hot Start II DNA Polymerase |
Reaction Speed | Fast |
Product Type | Hot Start High-Fidelity DNA Polymerase |
Shipping Condition | Wet or Dry Ice |
Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
13555160
|
Thermo Scientific™
F124S |
200 Reactions |
1302.00 SEK
200 reactions |
In Stock Log in to see stock. |
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13565160
|
Thermo Scientific™
F124L |
1000 Reactions |
5530.00 SEK
1000 reactions |
In Stock Log in to see stock. |
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Description
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase (Cat. No. EP0072).
The hot start modification of Phire Hot Start II DNA Polymerase is based on the Affibody inactivation technology. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.
Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Highlights
- Quick hot start—No reactivation step
- Fast enzyme—Amplify four times faster than with hot start Taq
- Robust—Minimal reaction optimization due to high inhibitor tolerance
- High yields—Abundant products due to high efficiency
- Longer PCR products—Amplify significantly longer DNA fragments than with any hot start Taq
- Direct loading on gel with Green Buffer
Applications
- Hot Start PCR
- Routine PCR
- Non-high fidelity PCR
- Fast PCR
- High throughput PCR
- Genotyping
Note:The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases. For optimal results start by accurately calculating your Tm with our Tm calculator.
Specifications
Medium | |
Fast | |
Wet or Dry Ice | |
2X | |
Blunt | |
DNA |
Phire Hot Start II DNA Polymerase | |
Hot Start High-Fidelity DNA Polymerase | |
Cloning, Hot-start PCR | |
Built-In Hot Start | |
Separate Components |
For Research Use Only. Not for use in diagnostic procedures.