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Invitrogen™ Phosphate Sensor

Phosphate Sensor

Brand:  Invitrogen™ PV4406

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Product Code. 10268302

  • 3330.00 SEK / Each
Estimated Shipment: 22-11-2024
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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.1μM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.
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Specifications

Specifications

Microplate Reader
Fluorescence Intensity
Phosphate Sensor is supplied as a 500 μM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C.
MDCC
10 nmol
430⁄450
Dry Ice
GTPases, ATPases, Phosphatases, Phosphodiesterases
Phosphatase Assay
Fluorescence
Phosphate Sensor
384-well plate
Kinetic
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For Research Use Only. Not for use in diagnostic procedures.