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Thermo Scientific™ Fluoraldehyde™ o-Phthaldialdehyde Reagent Solution (OPA)
Ready-to-use solution of o-phthalaldehyde, a fluorescent, amine-reactive, HPLC pre- or post-column labeling reagent to measure peptides or amino acids (Ex 340nm, Em 455nm).
Brand: Thermo Scientific™ 26025
This item is not returnable.
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Description
Thermo Scientific™ Pierce Fluoraldehyde (OPA) Reagent is a ready-to-use solution of o-phthalaldehyde, a fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.
Fluoraldehyde Reagent contains OPA (o-phthalaldehyde), which reacts with primary amines of amino acids, peptide and proteins to enable fluorescent detection and quantitation. The reagent that can be used as a protein or peptide assay reagent or as a pre- or post-column detection reagent for amino acid analysis (HPLC). Reaction of OPA with proteins and peptides yields linear results over a wide range of concentrations. The Fluoraldehyde Reagent Solution is supplied ready to use and enables fast quantitation of proteins or peptides in solution.
Highlights:
For pre- or post-column amino acid derivatization for fluorescent detection and quantitation
Reacts with all primary amine-containing analytes to yield fluorescent isoindole derivatives
Provides an accurate measure of both composition and absolute protein-peptide content
Ideal for work with recombinant proteins and synthetic peptides
Can be used for fluorescent protein or peptide assay
Pre-column derivatization mixtures can be injected into LC without any processing
Reagent Properties:
Formulation: 0.8mg/mL o-phthalaldehyde (OPA) in proprietary buffer, pH 10
Form: Clear, colorless to slightly yellow liquid
Reactive toward: Primary amines (–NH2)
Detection: Fluorescence (excitation/emission = 340nm/455nm)
Application: Pre- or post-column derivatization for detection by HPLC
Specifications
| o-Phthaldialdehyde | |
| Fluoraldehyde™ | |
| OPA (o-phthaldialdehyde) | |
| 945 mL |
| Amine | |
| Store under nitrogen at 4°C away from oxidizing reagents and direct sunlight. | |
| Fluorescence | |
| Liquid |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Fluoraldehyde o-Phthaldialdehyde Reagent Solution (OPA)
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
Hazard Statement
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H319-Causes serious eye irritation.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : potassium hydroxide,2-mercaptoethanol
For Research Use Only. Not for use in diagnostic procedures.