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Thermo Scientific™ Pierce™ Glutathione Superflow Agarose
Perform high-purity, large-scale purification of GST-tagged proteins with glutathione that has been immobilized to a crosslinked 6% beaded agarose.
Brand: Thermo Scientific™ 25236
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Description
Perform high-purity, large-scale purification of GST-tagged proteins with Thermo Scientific™ Pierce Glutathione Superflow Agarose, which consists of glutathione that has been immobilized to a crosslinked 6% beaded agarose.
Additional Information and Online Ordering
Pierce Glutathione Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates required for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemical and pH values, and it is compatible with common clean-in-place procedures. The specific binding of glutathione to the GST-tag results in high purity and high yield in a single step purification process.Highlights:
High dynamic binding capacity – binds 10mg of pure GST per mL of resin at a linear flow rate of 150cm/hour; can bind greater than 20mg/mL at 30cm/hour flow rate
High purity – provides greater than 90% purity when used to purify overexpressed GST or GST-fusion proteins from lysates
Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour
Compatible – maintains function after exposure to a wide variety of chemicals and pH values
Cost effective – resin is stable through multiple cycles of cleaning and reuse
Recommended for:
Medium- to large-scale FPLC purification of GST-tagged proteins
Specifications
| 10 mg Pure GST Binding per mL Resin at 150 cm/hour Flow Rate, 20 mg per mL at 30 cm/hour Flow Rate | |
| Pierce™ | |
| GST-Tagged Protein | |
| Glutathione | |
| Affinity, Agarose Resin |
| Upon receipt store at 4°C. | |
| 10 mL | |
| Agarose | |
| Bottle |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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