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Thermo Scientific™ Krypton™ Fluorescent Protein Stain
Stain proteins in polyacrylamide gels or transfer membranes for fluorescent detection with popular fluorescence imagers.
Brand: Thermo Scientific™ 46630
Description
Thermo Scientific™ Pierce Krypton Fluorescent Protein Stain enables sensitive fluorescent visualization (520nm excitation; 580nm emission) of proteins separated by polyacrylamide gel electrophoresis or on transfer membranes using popular commercial fluorescence imagers.
This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis (SDS-PAGE). Krypton Stain uses a protein-binding dye with fluorescence properties (Ex/Em = 520/580nm) suitable for visualization with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0.25ng per band in a typical mini-gel lane (greater than 2ng for the rapid staining protocol). Krypton Stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.Highlights:
Intense fluorescent stain – excitation and emission maxima of 520nm and 580nm, respectively (compatible with 532nm excitation light sources and 580 to 600nm emission filters)
Instrument-compatible – effective with popular laser-based and filter-based fluorescence imagers
Broad dynamic range – linear quantitative range spans three to four orders of magnitude
Sensitive – detect as little as 0.25ng protein with the standard protocol (approx. 2.5 hours); at least as sensitive as fluorescent stains from other suppliers
Fast – standard (2.5 hours) and rapid (30 minutes) protocols are significantly faster than procedures with other popular fluorescent stains
Versatile – compatible with proteomics workflows involving downstream mass spectrometry
Compact – supplied as a 10X solution to save storage space; just dilute with water before use
Specifications
Upon receipt store at 4°C. | |
Fluorescence | |
Protein Stain | |
500 mL |
In-Gel Detection | |
Proprietary Mix | |
Krypton™ | |
Protein |
Safety and Handling
- Krypton Protein Stain
Signal Word
- Warning
Hazard Category
- Skin corrosion/irritation Category 2
Hazard Statement
- H315-Causes skin irritation.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
Supplemental information
- MIXTURE LIST-Contains : benzaldehyde, aceticacid
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For Research Use Only. Not for use in diagnostic procedures.