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Thermo Scientific™ Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit
Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.
Brand: Thermo Scientific™ 44680
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Description
Thermo Scientific™ Pierce Mouse IgG1; Fab and F(ab')2 Preparation Kits use immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.
The kits use ficin protease to enzymatically digest the Fc portion of whole IgG to yield Fab or F(ab')2 fragments. Because the ficin protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free. An optimized procedure and high-performance desalting and Protein A spin columns make these kits easy to use.Highlights:
Enzyme-free digestion products – immobilized ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease
Optimized for mouse IgG1 – the kit procedure is specifically optimized for monoclonal mouse IgG1 antibodies, providing the best possible yield for this antibody subclass
High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time. Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG
Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments
Includes:
Ficin agarose resin, cysteine, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories
Specifications
| 10 Antibody Samples Each Containing 25 to 250 μg IgG | |
| Pierce™ | |
| Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG • Pierce Ficin Agarose, 0.8 mL • Cysteine-HCl, 1 g • Mouse IgG1 Digestion Buffer, 120 mL • NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns • Protein A Binding Buffer, 50 mL • IgG Elution Buffer, 50 mL • Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns • Spin Columns, 0.8 mL, 10 columns • Microcentrifuge tubes, 2 mL, 30 tubes Store at 4–8°C. |
For Research Use Only. Not for use in diagnostic procedures.