Thermo Scientific™ Pierce™ Protein L Magnetic Beads

Description

Perform antibody purification using manual or robotic magnetic separators with Thermo Scientific™ Pierce Protein L Magnetic Beads, high-capacity and high-throughput affinity particles.

Additional Information and Online Ordering

Recombinant Protein L, covalently attached to a blocked magnetic bead surface, selectively binds mouse and human antibodies through kappa light chains. The beads are commonly used to purify monoclonal antibodies in cell culture supernantants supplemented with bovine serum as Protein L does not bind bovine IgG. The Pierce Protein L Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms such as the Thermo Scientific™ KingFisher Instruments for high-throughput workflows.

Highlights:

• Selective – immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains

• Low non-specific binding – stable, pre-blocked beads provide clean purification of antibody

• Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific™ KingFisher Instruments)

Recommended for:

• Purification of monoclonal and polyclonal antibodies that bind poorly to Protein A or Protein G magnetic beads
• Purification of monoclonal antibodies from culture supernatants supplemented with bovine serum
• Protein L IP and co-IP assays
• Purification of ScFv and Fab fragments containing kappa light chains