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Thermo Scientific™ Pierce™ Renilla-Firefly Luciferase Dual Assay Kit
Simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.
Brand: Thermo Scientific™ 16185
1634.00 SEK valid until 2024-12-31
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Description
The Thermo Scientific™ Pierce Renilla-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.
The Pierce Renilla-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Green Renilla and Red Firefly luciferase activity. Green Renilla luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. This reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.Highlights:
Simultaneous – concurrent, filter-based, wavelength-separated detection of two luciferase activities
Sensitive – measure Green Renilla and red firefly luciferase activities in the same sample
Fast – no quenching step required, unlike in traditional sequential dual assays
Multiplex – capable of quantitating two cellular activities in the same sample(s)
Includes:
Cell lysis buffer, reaction buffer, and substrates coelenterazine and D-luciferin
Requires:
Green Renilla and Red Firefly luciferase reporters; Filter sets at 525nm±20nm for Green Renilla Luc and 615 to 675nm for Red Firefly Luc. Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time.
Recommended for:
Study two regulatory elements at the same time; Monitor two signaling pathways simultaneously; Enable studying more than one target per screen (e.g. off-target effects)
Specifications
| Assay Kit | |
| 100 Dual Luciferase Assays | |
| Sufficient For: 100 dual-luciferase assays in microplate wells • Renilla-Firefly Dual Assay Buffer, 5 mL (store at 4°C) • 100X Coelenterazine, 50 μL (store at -80°C) • D-Luciferin, 3 mg (store at 4°C) • Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature) Upon receipt, store kit at -80°C, or store individual components as indicated above. |
|
| Mammalian Cells | |
| Luminometer (Microplate) | |
| Enzyme Labeled | |
| Enhanced Chemiluminescence | |
| Pierce™ |
| Luciferase, Firefly Luciferase, Green Renilla Luciferase | |
| 100 Reaction Kit | |
| Reporter Enzyme, Luciferase Reporter Assay | |
| Bioluminescence | |
| 384-well plate, 96-well plate | |
| Chemical Substrate | |
| Luciferase Substrate | |
| Coelenterazine, D-Luciferin |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Pierce Renilla-Firefly Luciferase Dual Assay Kit
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 3
- Flammable liquid Category 2
- Specific target organ toxicity after single exposure Category 1
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H301-Toxic if swallowed.
- H311-Toxic in contact with skin.
- H331-Toxic if inhaled.
- H370-Causes damage to organs.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : methanol
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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