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Thermo Scientific™ Pierce™ Streptavidin Agarose Columns, 1 mL
Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.
Brand: Thermo Scientific™ 20351
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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price
Description
Thermo Scientific™ Pierce Streptavidin Resins comprise a wide selection of high-quality affinity beads and columns for nearly any kind of biotin-based purification procedure.
Additional Information and Online Ordering
Pierce Streptavidin Agarose and UltraLink Resins supply immobilized streptavidin biotin-binding protein in different resin formats, binding capacities and package sizes to suit nearly any immunoprecipitation or affinity purification procedure involving biotinylated molecules. The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11. The products are excellent choices for a variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies.Highlights:
Proven coupling chemistries – the methods used to prepare these resins are superior to typical cyanogen bromide (CNBr) immobilization, providing high functionality, low nonspecific binding and excellent leach-resistance
Versatile resins – Agarose (polysaccharide-based) and UltraLink™ Biosupport (acrylamide-based) resins provide two different options for particular experimental conditions and are compatible with gravity-flow, spin and FPLC methods
Available in multiple formats – choose from two resin types, regular or high capacity preparations, and small or large-scale package sizes (bulk sizes are also available)
Superior performance – High Capacity Resin has 2 to 3 times higher binding capacity than alternative supplier products, allowing use of small amounts of resin for experiments
Item-Specific Features:
- Agarose Resins are crosslinked 6% beaded agarose
- UltraLink Resins beaded acrylamide support (UltraLink Biosupport)
- Plus and High Capacity Resins have higher loading (i.e. density) of immobilized streptavidin protein than the regular forms
Recommended for:
Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies; Cell-surface labeling with biotinylation reagents, followed by precipitation with immobilized streptavidin; Purification of cell-surface glycoproteins using biotinylated Concanavalin A; Recovery of single-stranded DNA for dideoxy sequencing
Specifications
| 5 mL | |
| 45 to 165 μm | |
| 5 Columns | |
| Biotin-Tagged Molecule | |
| Streptavidin | |
| Spin Column |
| Contents: 5 × 1 mL prepacked columns with twist-off bottom tab and accessory pack containing five white tips. Storage: Upon receipt store at 4°C. Do not freeze the resin. Product is shipped at ambient temperature. |
|
| Pierce™ | |
| Recombinant | |
| Agarose | |
| Binding 15 to 28 μg Biotin Per Column (1 to 3 mg Biotinylated BSA Per Column) | |
| Affinity, Agarose Resin |
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Product Title
Thermo Scientific™ Pierce™ Streptavidin Agarose Columns, 1 mL > Columns, 5mL-capacity, 1mL resin; 5 columns
For Research Use Only. Not for use in diagnostic procedures.
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