Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)

Description

Invitrogen Platinum Hot Start PCR Master Mix, available in colorless and green formats, offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup. It retains all the features of Platinum Taq DNA Polymerase: specificity, robustness, and reliability.

Platinum Green Hot Start PCR Master Mix additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels.

Platinum Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. It is supplied with a separate vial of Platinum GC Enhancer, formulated for specific amplification and improved yields of GC-rich targets.

Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The antibodies dissociate during the initial PCR denaturation step, and DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity.

Features

• High specificity and increased yields with antibody-mediated hot-start PCR
• Convenient room temperature reaction setup
• Versatile formulation for broad range of amplicons
• Direct gel loading with green PCR master mix

Applications

• Hot Start PCR
• Genotyping
• Gene expression profiling
• Next-generation sequencing

Notes

• Platinum II Taq Hot-start DNA Polymerase is recommended superior PCR performance.
• For applications that require PCR product analysis by absorbance or fluorescence excitation, colorless format is recommended.