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Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

Brand: Invitrogen™ 14000013

2471.25 SEK valid until 2025-04-18
Use promo code "24107" to get your promotional price.

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Product Code. 15899844

3335.00 SEK / 200 reactions

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Includes: 4x1.25 mL Platinum II PCR Master Mix (2X), 2x1.25 mL Platinum GC Enhancer, 4x1.25 mL Water, nuclease-free

This item is not returnable. View return policy

Description

Invitrogen Platinum II Hot-Start PCR Master Mix (2X), available in colorless and green formats, offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase and leading hot-start technology. This master mix is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

Platinum II Hot-Start Green PCR Master Mix also includes two tracking dyes for direct loading of PCR products on gels.

Features

Innovative buffer enables universal annealing temperature by isostabilizing primer-template duplex structures
Engineered Taq DNA polymerase confers fast cycling and resistance to common inhibitors
Hot-start technology enables superior specificity, sensitivity, and yields and allows for room temperature reaction setup
Green PCR buffer reduces pipetting errors with direct gel loading

Applications

Amplification of DNA from complex genomic, viral, and plasmid templates
Amplification and improved yields of GC-rich targets
RT-PCR
Genotyping
High-throughput PCR

Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq DNA polymerase that shows increased resistance to reaction inhibitors originating from sample material or DNA purification steps. The polymerase has a higher DNA synthesis rate and delivers PCR results more than two times faster than other Taq DNA polymerases. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. With Platinum II Hot-Start PCR Master Mix (2X), different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified.

Notes

For applications that require PCR product analysis by absorbance or fluorescence excitation, colorless format is recommended.

Specifications

Content And Storage	• Platinum II PCR Master Mix (2X), 4 x 1.25 mL • Platinum GC Enhancer, 2 x 1.25 mL • Nuclease-free water, 4 x 1.25 mL Store at -20°C in a non-frost-free freezer.
GC-Rich PCR Performance	High
Reaction Speed	Fast or Standard
Quantity	200 reactions
For Use With (Application)	Hot-start PCR
Fidelity (vs. Taq)	1X
No. of Reactions	200 Reactions
Reaction Format	SuperMix or Master Mix
Starting Material	DNA

Format	Tube
Polymerase	Platinum II Taq Hot-Start DNA Polymerase
Product Type	Hot Start PCR Master Mix
Shipping Condition	Dry Ice
Concentration	2X
Hot Start	Built-In Hot Start
Overhang	3'-A
Size (Final Product)	5 kb or less
Color	Colorless

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Documents

Scientific Resources

Application Notes

Application Note: Multiplex PCR using Platinum II Taq Hot-Start DNA Polymerase File size: 372.7 kB

Application Note: Direct PCR from blood using Platinum II Taq Hot-Start DNA Polymerase File size: 394.1 kB

Flyers

Flyer: PCR enzymes and sample prep kits for microbiome research File size: 388.3 kB

Posters

Poster: Platinum II Taq Hot-Start DNA Polymerase: PCR simplified with universal annealing File size: 1 MB

Product Information

Manuals

User Guide: Platinum II Hot-Start PCR Master Mix (2X) Version: MAN0017535 Rev A (19 January 2018) File size: 1.1 MB

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2816764	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number14000013
Lot Number2816742	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number14000013
Lot Number2839816	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number14000013
Lot Number2842114	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number14000013
Lot Number2844717	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number14000013

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