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R&D Systems™ Recombinant Human BMP-4 Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Brand: R&D Systems™ 314-BP-01M
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Description
Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 3.00-30.0 ng/mL.Specifications
| Q53XC5 | |
| Bioactivity | |
| 652 | |
| BMP-4 | |
| Mouse myeloma cell line,NS0-derived human BMP-4 protein Ser293-Arg408 | |
| Disulfide-linked homodimer | |
| BMP2B, BMP-2 B, BMP2B1, BMP2BMCOPS6, BMP4, BMP-4, Bone morphogenetic protein 2 B, bone morphogenetic protein 4, DVR4, MCOPS6, OFC11, ZYME | |
| Recombinant Protein | |
| Human | |
| Reconstitute at 50 to 200 ug/mL in sterile 4 mM HCl containing (at least 0.1% human or bovine serum albumin.) |
| Bioactivity | |
| Lyophilized from a 0.2 um filtered solution in Acetonitrile and TFA with BSA as a carrier protein. | |
| M.W. (Observed): 19-24 kDa, reducing conditions 35-41 kDa, non-reducing conditions | |
| 1 mg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. | |
| <0.01 EU / 1 ug of the protein by the LAL method. | |
| BMP4 | |
| Unconjugated | |
| Recombinant | |
| 95%, by SDS-PAGE with silver staining. |
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