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Gibco™ RPMI 1640 Medium, low HEPES, low bicarbonate, no glutamine
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Brand: Gibco™ 42402016
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Description
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI 1640 is modified as follows:
- With: Phenol Red, HEPES
- Without: L-glutamine
The complete formulation is available.
Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Use
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Specifications
| ISO 13485 standards, FDA | |
| CO2 | |
| Animal Origin-free | |
| Mammalian Cell Culture | |
| RPMI 1640 | |
| Sterile-filtered | |
| No Glutamine, No Sodium Pyruvate | |
| Sustainable packaging | |
| 270–300 mOsm/kg | |
| Gibco™ | |
| Room Temperature |
| Storage: 2°C to 8° C, protect from light Shipping: Ambient Shelf life: 12 months from date of manufacture |
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| Roswell Park Memorial Institute (RPMI) 1640 Medium, 500mL, Liquid, 1 X Concentration, 7.0 to 7.4 pH, With Phenol Red, HEPES, 12 Months Shelf Life, 270 to 300mOsm/kg Osmolality, For Mammalian Cell Culture | |
| 1X | |
| Liquid | |
| Standard Serum Supplementation | |
| HEPES, Phenol Red, Sodium Bicarbonate | |
| Mammalian cell culture | |
| cGMP-compliant under the ISO 13485 standard | |
| 7 to 7.4 | |
| 500 mL |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.