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Cytiva Sepharose™ Fast Flow Cation Exchange Media

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Brand:  Cytiva 17-0729-10


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Regulatory: This product contains a substance listed in Annex XIV to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Product Code. 10576350

  • 940.00 SEK / 25mL
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Description

Description

Used for preparative protein separations in both research and industrial applications. Cytiva CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.

  • Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
  • Composed of crosslinked 6% agarose beads
  • Reproducible
  • Part of Sepharose Fast Flow ion exchange platform
  • High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
  • Available in range of pre-packed process development tools

SP Sepharose:

  • Ligand: Sulphopropyl
  • Ion Exchanger Type: Strong cation exchanger
  • Ionic Capacity: 0.18 to 0.25mmol H+/mL medium
  • Binding capacity: 70mg ribonuclease A/mL medium
CM Sepharose:
  • Ligand: Carboxymethyl
  • Ion Exchanger Type: Weak cation exchanger
  • Ionic Capacity: 0.09 to 0.13mmol Hl+/mL medium
  • Binding capacity: 50mg ribonuclease A/mL medium

Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals

TRUSTED_SUSTAINABILITY
Specifications

Specifications

25 mL
For Preparative Protein Separations in Research and Industrial AppliCatIons, for Capture and Intermediate PurifiCatIon of Wide Range of Approved Biopharmaceuticals
Strong CatIon Exchanger
70 mg Ribonuclease A/mL mediμm
400 to 700 cm/Hoμr
100 kPa
4 to 13
Sulphopropyl
Stable in commonly used aqueous Buffers: 1 M NaOH, 8 M Urea, 8 M Guanidine Hydrochloride, 70% Ethanol
4°C to 30°C
0.18 to 0.25 mM Hl+/mL
SP Sepharose Fast Flow Media
45 to 165 μm
6% Cross-Linked Agarose
20% Ethanol, 0.2 M Sodium Acetate
25 mL
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Safety and Handling

Safety and Handling

Product Identifier
  • SP Sepharose Fast Flow, 25 ml

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.

Precautionary Statement
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : ethanol
  • UNKNOWN ACUTE-18 per cent of the mixture consists of component(s) of unknown acute toxicity.

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