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Cytiva Sera-Xtracta™ HMW DNA Kit
High yield extraction of high-molecular-weight genomic DNA from blood, buffy coat, saliva, cultured mammalian cells and solid tissue with a simplified protocol.
Brand: Cytiva 29429140
Additional Details : CAS Number : 39450-01-6 Weight : 0.40000kg
Description
- Suitable for long-read sequencing: Isolates high-molecular-weight DNA > 200 kb.
- Efficient scale-up and flexibility: Provides robust chemistry for sample input volumes from 200 μL – 2 mL.
- Efficient removal of inhibitors to give high purity genomic DNA: Typical purity ratio (A260/A280 and A260/A230) of > 1.7.
- Automation friendly reagents and easy to use protocols
- Simplified protocol minimizes the co-purification of RNA.
- Compatible with molecular biology techniques, including next-generation sequencing (NGS), cloning, restriction enzyme digestion, PCR amplification and genotyping applications.
Specifications
Proteinase K 60 mg (dried); Lysis buffer 22 mL; Silica-coated magnetic beads 1.5 mL; Binding buffer 26 mL; Wash 1 buffer AQ 120 mL; Wash buffer 2 AQ 30mL; gDNA Elution buffer 14mL. Store at room temperature. | |
100 μL | |
96 Purifications |
Approx. 12 μg binding capacity based on specified 15 μL aliquot of supplied magnetic bead suspension = 1.5 mg beads at 100 mg/mL | |
Proteinase K 3mL, Lysis buffer 22mL, Silica-coated magnetic beads: 1.6mL, Binding buffer 26mL, Wash buffer 1 AQ 120mL, Wash buffer 2 AQ 30mL, gDNA Elution buffer 14mL | |
Purity ratio (A260/A280) > 1.7 |
Safety and Handling
- Sera-Xtracta Genomic DNA Kit
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P310-Immediately call a POISON CENTER/doctor/.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : guanidinium chloride, trometamol, proteases
- UNKNOWN ACUTE-70 per cent of the mixture consists of component(s) of unknown acute toxicity.
- UNKNOWN AQUATIC-Contains 70% of components with unknown hazards to the aquatic environment.