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IBA Lifesciences Strep-Tactin™ TACS Agarose Column
Gravity flow column suitable for traceless affinity cell and exosome isolation
Brand: IBA Lifesciences 6-6310-001
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Description
- Strep-Tactin™ TACS Agarose columns contain agarose macrobeads coated with the streptavidin variant Strep-Tactin™.
- A special crosslinking permits the use for cell isolation and depletion approaches.
- They were developed for column-based Traceless Affinty Cell Selection (Fab-TACS™/Nano-TACS™).
- In this approach, Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin™ on the agarose matrix.
- After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin™ Agarose by biotin addition.
- This way the cells are eluted ready for further downstream applications.
- The same principle is applicable for exosome isolation (Fab-TACS™ for exosomes).
- Alternatively to Fab-Streps/Nano-Streps, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding).
- This makes Strep-Tactin™ TACS Agarose columns also suitable for negative cell selection or depletion approaches.
Specifications
| 1 mL | |
| Pre-packed in buffer, pH 8: 100 mM Tris-HCl pH 8.0, 1 mM EDTA, 150 mM NaCl, 0.01% sodium azide | |
| Cross-linked agarose | |
| Affinity Chromatographic Cell or Exosome Isolation |
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