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Streptavidin, Streptomyces avidinii, Thermo Scientific Chemicals
Description
Streptavidin is used as a tetrameric protein used to visualize biotin conjugated molecules in ELISA. Due to the strong affinity of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin is widely used in molecular biology through its unique high affinity for the biotin.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
• Streptavidin is a 60-kDa extracellular protein isolated from Streptomyces avidinii, and it is considered a tetrameric protein with a very high affinity to biotin
• It has four binding sites for biotin and has the ability to bind four molecules of biotin
Application
• This compound has a strong affinity for biotin and biotinylated molecules, and because of this, streptavidin is considered one of the most important components in laboratory research kits. Due to its affinity for biotin, it is widely used in several molecular biology techniques, including ELISA, DNA purification, enzyme immobilization, and flow cytometry
• Streptavidin is used to visualize biotin-conjugated molecules in enzyme-linked immunosorbent assay (ELISA)
• Streptavidin has been used in microbubble preparation, which is an important technique used in research
• It has been used extensively in bionanotechnology to prepare beads and nanoparticles
• It is used to immobilize proteins and oligonucleotides
Specifications
Specifications
| Type | Streptavidin |
| Physical Form | Lyophilized Powder |
| Color | White |
| Quantity | 10 mg |
| Solubility Information | Gives a clear solution at 5mg/ml in 0.1M NaCl. is soluble in water at 1mg/mL,or in phosphate buffered saline (PBS). |
| Formula Weight | 55 kDa |
| Percent Purity | 95% |
| Chemical Name or Material | Streptavidin, Streptomyces avidinii |
RUO – Research Use Only