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Thermo Scientific™ Tango™ HRH3-bla U2OS DA Assay Kit

Brand: Thermo Scientific™ K1509

Product Code. 10277173

7300.00 SEK / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

The Tango™ HRH3-bla U2OS DA (Division Arrested) cells contain the human Histamine Receptor H3 (HRH3) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ HRH3-bla U2OS cells and the Tango™ HRH3-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of Methylhistamine. In addition, Tango™ HRH3-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

Specifications

Accession Number	NM_007232
Cell State	Division-Arrested Cells
Cytoplasmic Tail	Tail from AVPR2 GPCR
For Use With (Application)	GPCRs
Includes	HRH3-bla U2OS DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70 μg, Solution D, Tango™ HRH3-bla U2OS Assay Protocol, LiveBLAzer™FRET B/G Loading Protocol, Certificate of Analysis
Product Line	Tango™
Reporter Gene	BLA (Beta-Lactamase)
Type	Division-Arrested Cells Assay Kit
System Type	Tango™

Assay Entry	Cell-based beta-arrestin recruitment
Content And Storage	Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • Tango™ HRH3-bla U2OS DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • Tango™ HRH3-bla U2OS Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The Tango™ HRH3-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method	Fluorescence
Gene Symbol	HRH3
Population Status	Pool of Stable Cells
Quantity	1 Plate
Target Entry	HRH3, H3
Cell Line	U2OS
Druggable Target	GPCR

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Thermo Scientific™ Tango™ HRH3-bla U2OS DA Assay Kit

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