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Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/μL)

Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.

Brand:  Thermo Scientific™ EP0404

1747.94 SEK valid until 2024-09-17
Use promo code "21690" to get your promotional price.


Additional Details : Weight : 1.51950kg

Product Code. 10180780

  • 1948.00 SEK / 500 units
In Stock
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Description

Description

Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter.

Highlights

  • Thermostable—half life is more than 40 min at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Applications

  • Routine PCR amplification of DNA fragments up to 5 kb
  • High throughput PCR
  • DNA labeling

Notes

  • The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
  • The 10X Taq Buffer without Detergent is recommended for microarray experiments.
Specifications

Specifications

• Taq DNA Polymerase (1 U/μL), 500 μL
• 10X Taq Buffer with KCl, 2 x 1.25 mL
• 10X Taq Buffer with (NH4)2SO4, 2 x 1.25 mL
• 25 mM MgCl2, 2 x 1.25 mL

Store at -20°C.
Taq DNA Polymerase
Taq DNA Polymerase (recombinant)
Standard PCR
1X
3'-A
Low
Standard
500 Units
1 U/μL
No
Separate Components
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For Research Use Only. Not for use in diagnostic procedures.